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Flowjo 10 Manual
FlowJo meets FlowJo Portal. With FlowJo v10.5, you can now purchase and manage your individual license through the FlowJo Portal. Once you create your free account, you can use your FlowJo Portal ID to sign in to FlowJo anywhere, at any computer—no dongle or hardware address required. FlowJo™ Basic Tutorial Data Download. SeqGeq™ Basic Tutorial Download. Blog Newsletter Podcast Resources. Tutorials FlowJo Documentation SeqGeq Documentation Grant Resources Documents Flow Cytometry News FlowJo Africa FlowJo and BD are one! FlowJo is your biggest fan and strives to be an outstanding source of support. We're here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to results―one cell at a time. 当前位置：电脑软件  行业软件  健康医药  flowjo 10 破解版 V10.0.6 flowjo 10 破解版 V10.0.6 大小：131MB 语言：英文 类别：健康医药.
Please find below a version history of FlowJo vX (version 10). https://trueoload489.weebly.com/textfridge-1-2-123movies.html.
10.0.5 (Released December 6, 2012) Doyourdata start menu professional 3 9 free. Does office work on macbook.
Fixed:
Boolean Not gates
Opening the Matrix Editor
Canceling a Derived Parameter
Compensation opening in a saved ACS file
Compensation and reagent names are now saved in a WSP file
Compensation on Guava files
Influx files now load
Tint for gates in the GW
Bisector gates can now be created on samples with the same stain
Synchronized gates now work with magnetic gates
Gates disappearing on log parameters when moved
Can now add more than one sample to a grid at a time
FlowJo now installs on Windows 8
Internationalization flag has been removed; please change language through preferences
Deleting layouts in the Layout Editor
Layout Editor iteration with controls, stats, keywords has all been fixed
Printing in the Workspace and the Layout Editor
Robust Standard Deviation is now an available statistic
Freq. of…statistics are now correct when dragged to the Layout Editor
Headers/Footers now work in the Table Editor
Can now edit columns in the Table Editor without changing defaults
Custom names now work with Keywords in the Table Editor
Can now make correlations and line plots of keywords and formulas in the Table Editor
Can now create and edit statistic boxes in the Layout Editor
FJML is now accessible by double clicking in the Layout Editor
Dragging children gates to groups now propagates correctly
Can now copy from the right click menu in the workspace
Can now save group export in a template and run via command line
Click points for disclosure triangles are now on center
Known Issues:
Collection time does not sort in the workspace
Lists disappear when scrolled in W7; Java 7 issue only
Redo issues with group applied gates
Working on formatting issues in text boxes
Correlation/line plots are not correct using the buttons. Use right click to make plots in a batched table
When printing from the GW, it uses the Layout Editor as a canvas
Preferences for header/footers are not working; do work in the platforms
Top of Offset Histograms are cut off in the Layout Editor with many samples
Cannot append to a PPT presentation
Save as PDF has output issues. Best to batch to PDF
Adding backgating and MGOs to multiple plots only adds to one
Save graphic as SVG does not produce a scalable graphic
Statistics on bisector gates are in the middle of the plot
Loading WSP saved in a different language
Ancestry, backgating, and combination gates are not working when loading JO files
Compensated parameters on Miltenyi FCS3.1 files
3D is not responding
Up uploader for instagram 3 10 2 download free. 10.0.4 (Release date: October 5, 2012)
Fixed:
Compensation Matrix Editor:Double clicking the Badge of a sample throws a stack
Matrix Editor dialog tooltip issues
Not able to manual change values in an applied matrix
Compensation:Compensating on Comp group throws a stack
Resetting assignments does nothing after clear
Comp sample assignment list and positive drop down list is disabled when re-selecting it
Compensation dialog tooltip issues
Double clicking a compensation node (if shown) doesn't reopen editor
Unstained control displayed as log on biex plot in CE
CE splits positive histograms as positive and negative controls
Live stain still shows compensation after compensation and parameter can't be found
Detection of the unstained negative
.jo file opening:Refresh issue where stats show 0 in a batched table
Overlapping charts
FlowJo 7.6.5 .wsp file opening:Compensation matrix reading
GW sizing
Gate statistics refresh
Compensation control sample loading
Sample reconnection
Loading compensated parameters
Saving grids and ancestry alignment in Layout Editor
File Formats:Cyntellect and ADVIA 120 8-bit FCS2.0 file handling
FCS 3 matrix export
Graph Window:Gate name dialog position on PC
Copy Image from GW paste
Changing the Plot Type and opening a child population causes the GW to expand
Copy Image With and Without Transparency on a child population
Gate anchor point positioning
Internationalization:Restarting in a foreign language
Excel and graph window formatting of Chinese and Japanese characters
Comma formatting for decimal place lost upon excel export
Layout Charts:NxN plots with many parameters
Coloring in the LE affects the border and Legend
Outliers do not stay checked
Refresh issue with graph changes
Layout Editor:Dragging a sample to the LE throws a stack
Guides
Right click Axis menu
Canceling a Batch in the LE
Copy and Paste between separate layouts
Layout Outputs:PNG, JPG saving
Image copying and pasting
Text boxes in PNGs
Layout Overlays:Count for Auto scale
Large dots 
Adding statistics to the gated population in the Legend
Movies: making movies by Event #
Preferences:Lin/Log scaling saving
FACSCalibur not showing log parameters
Gallios preference added to switch between FCS3.0 and FCS 2.0 scaling ($PnR)
Show Header/Footer in Tables
Add % sign text is cut off in prefs (Mac)
Adding a new cytometer preference
Recognizing Cytof data
Setting Width basis for FACSDiva-generated data
Graph preferences: Contour Levels, Show Outliers
Printing:Print preview through the GW
Multiple improvements to Batch to printer in the LE
Statistics: Group owned Statistics in the WS refresh issue
Table Editor:HTML headers alignment
Resizing Columns
Row deletion order
Undo/Redo:Deleting a Sample in Compensation group after Compensating
Compensation window Undo
Remove Layer from the Layout Legends
File organization after drag and drop
Ordering of new group and newly added sample undos
GW sizing after Undo
Grids in the LE
Workspace:Creating a New Group during a file load
Saving Customized Ribbons
Responsiveness during gate/statistic calculation
Help menu access
Exporting gated population
Statistic refresh issue when Compensation dialog open
Default Font size
10.0.3 (Release date: August 6, 2012)
Thanks to the user feedback, the following list of user-found defects has been fixed in this release. FlowJo also makes every attempt to automatically notify us when there are defects, and we will continue to monitor both the automated reports and user-sent ones from Tech Support to continue improving this release.

Fixed:
3D viewer now opens from the Table Editor
Not gates of a not gate show correct statistics
Applying an edited matrix moved data in nested gates
Can now apply v9 matrix file with all extensions .mtx, .txt. and the default .txt
Reset in the Matrix Editor now resets values
Right clicking compensation badges in the workspace to remove and edit now work
Compensation now works with Sortware and Cyan data
Able to apply Derived Parameter to another sample
Loading JO files fixed binary warning, legends not showing, and overlays losing a layer
Loading Workspace (WSP) files fixed Layout Editor, comp group losing samples, reconnecting of files if in the same folder automatically, page breaks, and zoom are now saved correctly and don't reset
Can now read Stratedigm data
Fixed jumping gates on transformed data
Manual percentage gates are now showing in the workspace
Events on axis warning is now consistant
X axis parameters are now correct when showing graph in excel
Curly Quads disappearing and ability to drag off screen are fixed
PDF: Chinese characters now show, batching no longer clears the layout
Resizing a graph in the LE ruined the batch; now fixed
Rulers now update when page breaks are moved in the Layout Editor
Web Animation is now working in the Layout Editor
PowerPoint is now working
Copy and pasting out of the Layout Editor now has a regular border
Can now save text boxes as EMF
New Serial Number license screen bigger and better and can now be saved in preferences
Keyword combination for file name fixed
Repositories are now saving usernames in preferences
Printing now follows page breaks
Frequency of…can now be specified in the Table Editor and in the workspace
Formulas with column references now work in the Table Editor
Templates now save the control and overlays
Undo/Redo fixes in the Workspace, Compensation, Graph Window, Layout Editor
Able to drag gates and statistics to another workspace or sample
Text wrapping in Annotate Experiment now works
Flowjo 10 0 2Known Issues:
HTML headers are misaligned
Keywords are not saving in templates
Shortcut keys for 3D viewer do not work
Sample Quality is not fully implemented
Cyan and FACSCalibur data is not showing log parameters; has to be manually adjusted
Append to PowerPoint has an error and is not working
Saved Workspace and JO files still has layout issues with MGO's and refresh issues
Mac laptop mouse not working. 10.0.2 (Release date: July 12th, 2012)

Fixed:
3D viewer now opens from the Table Editor
Not gates of a not gate show correct statistics
Applying an edited matrix moved data in nested gates
Can now apply v9 matrix file with all extensions .mtx, .txt. and the default .txt
Reset in the Matrix Editor now resets values
Right clicking compensation badges in the workspace to remove and edit now work
Compensation now works with Sortware and Cyan data
Able to apply Derived Parameter to another sample
Loading JO files fixed binary warning, legends not showing, and overlays losing a layer
Loading Workspace (WSP) files fixed Layout Editor, comp group losing samples, reconnecting of files if in the same folder automatically, page breaks, and zoom are now saved correctly and don't reset
Can now read Stratedigm data
Fixed jumping gates on transformed data
Manual percentage gates are now showing in the workspace
Events on axis warning is now consistant
X axis parameters are now correct when showing graph in excel
Curly Quads disappearing and ability to drag off screen are fixed
PDF: Chinese characters now show, batching no longer clears the layout
Resizing a graph in the LE ruined the batch; now fixed
Rulers now update when page breaks are moved in the Layout Editor
Web Animation is now working in the Layout Editor
PowerPoint is now working
Copy and pasting out of the Layout Editor now has a regular border
Can now save text boxes as EMF
New Serial Number license screen bigger and better and can now be saved in preferences
Keyword combination for file name fixed
Repositories are now saving usernames in preferences
Printing now follows page breaks
Frequency of…can now be specified in the Table Editor and in the workspace
Formulas with column references now work in the Table Editor
Templates now save the control and overlays
Undo/Redo fixes in the Workspace, Compensation, Graph Window, Layout Editor
Able to drag gates and statistics to another workspace or sample
Text wrapping in Annotate Experiment now works
Flowjo 10 0 2Known Issues:
HTML headers are misaligned
Keywords are not saving in templates
Shortcut keys for 3D viewer do not work
Sample Quality is not fully implemented
Cyan and FACSCalibur data is not showing log parameters; has to be manually adjusted
Append to PowerPoint has an error and is not working
Saved Workspace and JO files still has layout issues with MGO's and refresh issues
Mac laptop mouse not working. 10.0.2 (Release date: July 12th, 2012)
Fixed:
3D Viewer: Ability to drag 3D node to LE
Boolean Gates:Getting a replace node dialog when trying to create AND gate
Boolean gating is not working – refresh issue
Comp Matrix Editor:Unchecking all the Parameters after unchecking Show All in ME throws a stack
ALLBY in ME
Document:X icon gets fuzzy when minimized – Mac only
Add Samples to also reconnect broken ones
Graph Window:Curly Quad gate lines is showing incorrect shape at the end of the lines
GW gate annotation copies the background color in the GW
GW transposed axis does not work
GW shrinks when Reset This Graph Window's Size and Reset All GW Size is selected
Manual percentage gate on a histogram throws a stack and doesn't work
Able to drag curly off screen and crash FJ
Help button in Gate Name dialog links to a Page Not Found
Child populations GW's size is stretched out
Installation: Version number needs to show in finder/explorer
Layout Charts:Right clicking on an axis in the LE to change to axis is not working
NxN plots are very small on view
String name shows in Graph def for lock ratio
Adding a legend from the right click menu in the LE is blank
Layout Editor:Compensation Matrix: Name Keyword in LE Text boxes/stat box is blank
Insert Annotation through FJML in text boxes is showing error
Remove look/feel options from the LE – causing lots of stacks and problems
Highlighting in the figure presets box is too dark – can't see text
Layout Iteration:Parameter not found then changing graphs when viewing NxN
Movies:GW Movie showing same display for every frame past the first
Some frames show blank in a movie
Population Comparison: Stack when adding all samples to control
Preferences:Legend Palette in the Annotation Prefs is not working
Gradient Color in the Layouts prefs does not work with grids
Set Stroke (Line Weight) pref is duplicated
Set Stroke (Line Weight) from Annotation prefs does not work
Repositories: Cancel out of Available Repositories throws a stack
Sample Quality: Check sample quality not working on a group
Shortcut Keys:Shortcut key to align bottoms in the LE is not working
Send Backward and Bottom icons in the LE have the same shortcut key: Ctrl + B
Table Editor: Batched table has string name issuesTransforms are only being applied to one sample and not the group
Linear/Log parameter lists are now correctly wired in
Workspace:Annotation experiment needs text wrapping in all boxes
Alt-drag loses its ability to drag all after dragging to the group
Creating multiple groups keeps the same default color
Not able to click into some boxes in Annotate Experiment
Annotate experiment does not save information
10.0.1 (Release date: June 21, 2012)
SSC-W showing log on linear data
Exporting to CSV on PC
Directions on how to reconnect file from a JO or WSP file
Better offline help dialogs
Better handling of statistics when group owned and deleted
Movie generation was improved
'Hide axes numbers' in LE charts will reposition axes labels closer to the plot
Known issues:
Manual range gates cannot be created
NxN pots display too small
Calculate Width basis
Flowjo 10 0 Error10.0.0 (Release date: June 13, 2012)
New Features:
Flowjo 10 ManualWorkspace Features:Ribbons: Customizable, fluent user interface. Tailor FlowJo for your analysis workflow
Split Group Panel: Able to see more detailed information about groups and their gates
Redesigned Compensation Editor: Automated Compensation, easier to read and analyze
International Controls: Chinese, German, French, Japanese, and Spanish are all included. Localization efforts will continue until FlowJo knows how to speak your native language
FCS Scan: Search the contents of any folder or drive for FCS files and quickly sort the contents by FCS keywords to find the files that match your experimental requirements
Workspace Find / Replace: Change keyword values in the workspace across an entire data set quickly and easily
MIFlowCyt Annotation: Antibody panel annotation is a great way to ensure the integrity of your experimental design and bolster your journal submissions
SOPs & URL bookmarking: Drag a URL (web address) from your browser to the bottom of the workspace window and it will show up as an icon for easy reference to websites or SOPs (Standard Operating Procedures)
Badges / Sample Quality Check: FlowJo now checks your samples at load time for outliers of parameters vs Time looking for drifts in the signal
Cytometer Preferences: V10 makes smart decisions about data display based on your cytometer
Gate Window Features:Breadcrumb Bar Navigation: Able to see all gate's names and their hierarchy in the graph window
Parameter Transform Editing: New interface, easier to use, new preview screen
Curly Quads and Spider Quads: Curved quad gates allow for actuate gating on compensated parameters
Tethered Gates: Gates linked to population density
Table Editor Features:Ribbons: Customizable, fluent user interface. Tailor FlowJo for your analysis workflow
Plots (Time Series, Correlation, Line, 3D): New plots to help visualize data based on time, correlation, and in 3D space
Range based formatting: Ability to set range formatting in preferences, add new ranges, use default ranges, delete ranges, and control how these appear in the Table Editor
Layout Editor Features:Ribbons: Customizable, fluent user interface. Tailor FlowJo for your analysis workflow
Locks (Position, size, binding): Able to lock objects in the Layout Editor so editing other objects will not have effects
New Properties (Shadow, gradient fill, rotation): New object properties that turn the Layout Editor into a drawing program. Able to add graphic features to any object in the Layout Editor
New Drawing Tools ( RoundRect, Diamond, Triangle, Ellipse): New drawing tools to help highlight analyses
Rulers: Helpful to organize layouts
Drag guides: Helpful for layouts that need publication quality organization. Guides help keep your layout objects precisely in line
Rich text boxes: Allow editing and formatting of font/text size and color. Can format and link to data in the workspace
Equations in text boxes: FJML gating and sample annotation can now be displayed inside of rich text boxes
Sample/Population Based Statistical Tables: New statistic table in the Layout Editor that allows drag/drop of samples and gates, statistics, and adding of new statistics and annotations to the table
Improved Grids: Rich text and a much improved mechanism for displaying plots, stats, and text in the Grid Tool have made it a more powerful mechanism for displaying plate based reports
URL link box: Many times it is helpful to display images or web based reports in-line with new data. FlowJo can display web pages or images from the internet in the Layout Editor so that you can more easily reference previously acquired data or reports pertinent to your current experiment
Invisible Features:Command Line Interface: Create batches and high throughput analyses via command prompt or terminal
Repository Support: Able to access repositories through FJ to download and upload data and workspaces
Remote and distributed engine support: Is your computer slow? Remote and distributed engines allows users to utilize multiple engines on our local server or their local servers to speed up analysis in FlowJo
Destinations attached to template reports: Direct your workspace and report saving to user-set file destinations, to control and protect your frequently-run analyses